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KMID : 0894520060100040239
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Development & Reproduction
2006 Volume.10 No. 4 p.239 ~ p.245
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Effect of β-Mercaptoethanol Supplement during In Vitro Maturation on IVM, IVF and Glutathione Level in Bovine Oocytes
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Oh Shin-Ae
Chung Yung-Chai
Kim Chang-Keun
Pang Myung-Geol
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Abstract
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Experiments were conducted to determine the effects of beta-mercaptoethanol(β-ME) supplements to the maturation medium on in vitro fertilization(IVF) and intracellular glutathione(GSH) concentration. Bovine cumulus-intact oocytes were matured in TCM-199 medium containing FBS, hormonal supplements, and β-ME(0, 25 and 50㎛) for 12h and 24 h. After culture, cumulus-free matured oocytes were co-incubated with frozen-thawed spermatozoa for 24h. Maturation rate increased(p<0.05) in β-ME treatment group, but no significant differences among treatment groups. Also, increases(p＜0.05) in intracellular GSH concentration before and after fertilization were observed in 50㎛ β-ME supplements to the maturation medium. Male pronuclear formations after IVF was increased(p＜0.05) in β-ME treatment group, but no significant difference among treatment groups. In conclusion, supplementing β-ME into the maturation medium increased maturation rates, fertilization rates, and intracellular GSH concentrations.
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KEYWORD
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β-Mercaptoethanol, Glutathione, IVM, IVF, Bovine oocyte
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